Fig. 3 | Scientific Reports

Fig. 3

From: Characterization of a new mutation of mitochondrial ND6 gene in hepatocellular carcinoma and its effects on respiratory complex I

Fig. 3

mtDNA-encoded Complex I proteins are overexpressed but not efficiently assembled in tumor mitochondria. (A) Representative Western blot analysis of 40 µg of mitochondrial extract from distal and tumor tissues separated on SDS-PAGE. The expression of nuclear DNA (nDNA)-encoded proteins reduces the mitochondrial DNA-encoded proteins that are upregulated in tumor mitochondria. In the graph are reported the means and standard deviations of three independent replicates expressed as relative to distal tissue (*** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05). (B) Normalized amounts of BN-PAGE resolved Complex I were isolated and separated on SDS-PAGE to evaluate the levels of mtDNA-encoded proteins for both Pt_ND6-WT and Pt_ΔND6.

Back to article page