Fig. 2
PCR-Based Identification of Hba-a1 (E1-3) and Hba-a2 (E1-3) Allele-Targeted Fetal Mice. (A) The diagram illustrates the strategic placement of two PCR primer pairs utilized for genotyping. (B) PCR outcomes for Hba-a1 (E1-3) allele-targeted fetuses, amplified with Hba-a1:F1R1 primers, where wild-type and double knockout alleles yield bands of 659 bp and 0 bp, respectively. (C) PCR results for the same Hba-a1 (E1-3) allele using Hba-a1:F2R2 primers, exhibiting band sizes of 390 bp for wild type and 0 bp for double knockouts. (D) PCR screenings for Hba-a2 (E1-3) allele-targeted fetuses using Hba-a2:F1R1 primers, with band sizes of 538 bp for wild type and 0 bp indicative of double knockouts. (E) PCR outcomes for Hba-a2 (E1-3) allele knockouts utilizing Hba-a2:F2R2 primers, differentiating wild type (491 bp) from double knockouts (0 bp) bands. Note: Numbering System: The first digit in the number (e.g., 1–1) represents the pregnant mouse’s ID, and the second digit represents the offspring’s ID. For example, 1–1 and 1–2 refer to the first and second offspring of pregnant mouse 1, respectively. This pattern applies to all other numbers. WT wild type control, N null control, M marker.
