Fig. 4 | Scientific Reports

Fig. 4

From: In vivo transplantation of mammalian vascular organoids onto the chick chorioallantoic membrane reveals the formation of a hierarchical vascular network

Fig. 4

Dextran injections show blood flow connections between the chick host and organoid. (A) Sketch of the injection protocol. After CAM implantation for 11 days, a chick vein was injected with 3 kDa fluorescent dextran. Created with Biorender.com. (B) Image of PECAM-1 labeled organoid perfused with dextran after injection. Dextran is present within the endothelial network, indicating a blood flow connection between the chick embryo host and engrafted organoid. (C-C’’ and D-D’’) Higher magnification of boxed areas in (B) depicts dextran sequestered within the organoid endothelial network.

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