Fig. 3 | Scientific Reports

Fig. 3

From: Cancer cells impact the microrheology of endothelial cells during physical contact or through paracrine signalling

Fig. 3

Representative confocal images of endothelial cells. (a) Control ECs versus ECs cultured in T24-ECGM, (d) Control ECs vs. ECs in contact with cancer cells. Cells were stained to localize actin fibers (Phalloidin, red) and nuclei (Hoechst, blue). Actin–GFP transfected T24 cancer cells were used to localize the sites of contact with ECs (green). Normalized total area of actin fibers (\(\frac{A_f}{A_e}\)) and clusters (\(\frac{A_c}{A_e}\)) for ECs (b, c) cultured in T24-ECGM and (e, f) ECs in-contact with CCs. Scale bar = 20 μm.

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