Fig. 3

Chromatograms illustrate the specific molecular mass of unextended primers (UEP) and single-base extension (SBE) products corresponding to target genes, gdh, recN, and cps. The presence of SBE products with molecular masses corresponding to the gdh and recN genes indicates the presence of S. suis. Meanwhile, distinct molecular masses of SBE products for the cpsJ and cpsK genes can differentiate between specific serotype pairs: serotypes ½ and 2, as well as serotypes 1 and 14. (A) S. suis serotype ½; (B) S. suis serotype 2 reference strain P1/7; (C) S. suis serotype 1; (D) S. suis serotype 14. The x-axis represents the molecular mass and the y-axis represents the peak intensity.