Table 1 The information of primers designed.
S. suis serotype | Primer name | PCR primer (FP, RP) | PCR amplicon (bp) | Extension primer (EP) | Molecular mass (Da) | |||
|---|---|---|---|---|---|---|---|---|
Tm (°C) | GC (%) | Tm (°C) | GC (%) | UEP | SBE product | |||
1, 14 | cps1J/14J | 68.4, 67.8 | 46.7, 43.3 | 132 | 50.0 | 45.0 | 6164.00 | 6491.10 |
½, 2 | cps½/2J | 69.1, 66.8 | 46.7, 41.4 | 168 | 49.1 | 50.0 | 5653.70 | 5940.90 |
3 | cps3L | 70.5, 68.1 | 50.0, 41.9 | 195 | 50.2 | 25.0 | 8663.70 | 8990.80 |
4 | cps4M | 70.5, 67.6 | 50.0, 43.3 | 156 | 49.0 | 29.2 | 7282.80 | 7570.00 |
5 | cps5N | 70.0, 70.9 | 46.7, 50.0 | 140 | 58.5 | 54.5 | 6652.40 | 6979.50 |
6 | cps6I | 70.3, 69.5 | 50.0, 46.7 | 177 | 47.6 | 44.4 | 5561.60 | 5888.70 |
7 | cps7H | 72.2, 70.6 | 50.0, 50.0 | 119 | 57.4 | 45.8 | 7244.80 | 7492.00 |
8 | cps8H | 69.3, 71.2 | 46.7, 50.0 | 149 | 50.4 | 40.0 | 6064.00 | 6311.20 |
9 | cps9H | 70.3, 70.5 | 45.2, 50.0 | 153 | 54.5 | 44.0 | 7740.10 | 8011.30 |
10 | cps10M | 69.2, 71.3 | 43.3, 50.0 | 155 | 49.9 | 56.2 | 4978.30 | 5265.50 |
11 | cps11N | 70.2, 71.4 | 50.0, 50.0 | 156 | 51.0 | 33.3 | 7311.80 | 7638.90 |
12 | cps12J | 69.5, 70.2 | 50.0, 45.2 | 149 | 46.7 | 47.1 | 5186.40 | 5473.60 |
13 | cps13L | 70.3, 69.8 | 46.7, 50.0 | 154 | 48.7 | 34.8 | 7136.70 | 7463.80 |
15 | cps15K | 69.1, 67.9 | 46.7, 38.7 | 140 | 47.0 | 18.5 | 8256.40 | 8527.60 |
16 | cps16K | 70.4, 70.6 | 50.0, 50.0 | 150 | 48.0 | 42.1 | 5683.70 | 5954.90 |
17 | cps17O | 71.9, 68.9 | 50.0, 43.3 | 162 | 53.0 | 58.8 | 5303.50 | 5574.70 |
18 | cps18N | 68.4, 68.9 | 43.3, 46.7 | 119 | 54.9 | 62.5 | 4914.20 | 5241.30 |
19 | cps19L | 69.2, 69.8 | 46.7, 46.7 | 134 | 54.0 | 66.7 | 4467.90 | 4715.10 |
21 | cps21P | 66.5, 68.8 | 41.4, 43.3 | 178 | 47.4 | 31.8 | 6718.40 | 6965.60 |
23 | cps23J | 70.5, 68.4 | 50.0, 43.3 | 187 | 50.8 | 30.8 | 7916.20 | 8187.40 |
24 | cps24M | 69.7, 69.6 | 46.7, 50.0 | 191 | 46.0 | 36.8 | 5907.90 | 6195.10 |
25 | cps25M | 69.6, 70.9 | 46.7, 50.0 | 182 | 48.0 | 35.0 | 6221.10 | 6548.20 |
27 | cps27K | 70.0, 71.2 | 46.7, 50.0 | 150 | 48.4 | 50.0 | 4842.20 | 5089.40 |
28 | cps28L | 69.0, 69.5 | 46.7, 43.3 | 144 | 47.4 | 31.8 | 6816.50 | 7063.70 |
29 | cps29L | 71.2, 71.8 | 50.0, 50.0 | 167 | 51.3 | 36.4 | 6761.40 | 7088.50 |
30 | cps30I | 69.2, 71.0 | 46.7, 50.0 | 132 | 56.0 | 42.3 | 8097.30 | 8424.40 |
31 | cps31L | 70.4, 68.0 | 46.7, 41.9 | 169 | 47.7 | 44.4 | 5526.60 | 5797.80 |
½, 1 | cpsK | 71.0, 69.4 | 50.0, 46.7 | 158 | 47.3 | 17.9 | 8573.60 | 8844.80 |
2, 14 | cpsK | 71.0, 69.4 | 50.0, 46.7 | 158 | 47.3 | 17.9 | 8573.60 | 8820.80 |
All S. suis | gdh | 70.5, 68.9 | 45.2, 38.2 | 225 | 51.8 | 33.3 | 7409.90 | 7681.10 |
All S. suis | recN | 69.8, 72.4 | 45.2, 51.6 | 191 | 55.3 | 43.5 | 7021.60 | 7348.70 |
