Fig. 7
Sensitivity of 2ndpHluorin. (a). Traces showing fluorescence intensity changes, (expressed as fluorescence normalized to the baseline signal) upon stimulations with 1, 5, 10, 20 or 40 APs. (b) Quantification of fluorescence intensity across synaptic boutons. An arbitrary value of 1 was assigned for the baseline (‘Base’), and this value was compared to all stimulation paradigms. The following number of synaptic boutons per condition were analyzed: 1 AP = 79 synapses, 5 APs = 82 synapses, 10 APs = 108, 20 APs = 112, 40 APs = 135, N = 5 independent experiments. Each black circle represents the mean value per experiment. Each grey circle represents a synaptic bouton. Data are presented as a box plot showing the median and quartiles, while whiskers represent the full data range. Statistical analysis was performed using one-way ANOVA, followed by Tukey’s multiple comparisons test.
