Fig. 4 | Scientific Reports

Fig. 4

From: Molecular mechanisms and therapeutic targets in glioblastoma multiforme: network and single-cell analyses

Fig. 4

Single-cell RNA sequencing (scRNA-seq) analysis of blue module subnetwork genes revealed their roles in pericytes, endothelial cells, and glioma cells. (A) Uniform manifold approximation and projection (UMAP) plots of 138,002 aggregate single cells from 24 samples. The clusters were identified via the UMAP algorithm. Each cluster is numbered and color-coded to represent different cell populations. (B) All nine cell clusters in glioma were annotated with “singleR” and CellMarker according to the composition of the marker genes. (C) Violin plot showing the expression of blue module subnetwork genes in nine cell clusters. (D) Dot plot presenting the expression of blue module subnetwork genes in nine cell clusters. (E) Heatmap displaying the expression of blue module subnetwork genes in low-grade glioma (LGG) and newly diagnosed glioblastoma multiforme (GBM) (ndGBM), as well as in nine distinct cell clusters. (F–H) Violin plot showing the expression of blue module subnetwork genes in pericytes (F), endothelial cells (G), and glioma cells (H) in patients with LGG or ndGBM.

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