Fig. 5
Validation and prognostic significance of COL1A1, COL4A1, and VIM expression in GBM tissues, (A,B) Univariate (A) and multivariate (B) Cox regression analyses of risk scores and clinical characteristics. (C) Nomogram model construction. (D) Calibration curve of the nomogram. (E) mRNA expression levels of COL1A1, COL4A1, and VIM in paracancerous and tumor tissues of patients with glioblastoma multiforme (GBM). The data are from five replicates. (F–G) Immunohistochemical analysis and statistical analysis of COL1A1, COL4A1, and VIM expression levels in paracancerous and tumor tissues of patients with GBM. Scale bar, 50 μm. (H–I) Representative confocal images for GFAP, VIM, α-SMA, COL1A1, CD31, and COL4A1 of human tissues (n = 3) from paracancerous and tumor regions. A minimum of 5 randomly chosen fields of each tumor (n = 5) were evaluated. Scale bars, 50 μm.
