Fig. 4
The effects of the mTORC1 inhibitor rapamycin on the regulation of tianeptine-induced autophagic proteins expression in B27-deprived hippocampal cells. B27-deprived cells were incubated with rapamycin (RAP, 2 µM) for 30 min prior to treatment with tianeptine (TIA; 100 µM). Following a three-day incubation period, Western blotting was conducted to assess the levels of mTORC1 phosphorylation (n = 6) (A), the LC3B-II/I ratio (n = 6) (B), and p62 (n = 6) (C). Quantitative analyses were normalized to the total mTORC1 or the α-tubulin band. Full-length uncropped gels for all Western blots are available in the Supplementary Information. The values are expressed as the mean ± standard error of the mean (SEM) and represent the percentage of the control (B27−, no drug) values. **p < 0.01 versus control; †p < 0.05 and ††p < 0.01 versus tianeptine-only-treated cells.
