Fig. 6
From: The m6A reader IGF2BP3 promotes HCC progression by enhancing MCM10 stability
MCM10 had Potential to be an Indicator of IGF2BP3 modulation. (a) The Venn plot showed MCM10 was identified based on the intersection analysis in TCGA data. (b-c). The expression of MCM10 between normal and tumor tissues in TCGA data. (d) The survival analysis of HCC patients with low and high MCM10 expression in TCGA data. (e) The potential m6A sites in MCM10 were predicted by SRAMP. The different colored lines indicate different confidence levels. (f) The mRNA of MCM10 was enriched by the m6A-specific antibody compared to IgG in HCCL-M3 and Huh-7 cells. (g) The mRNA of MCM10 was enriched by the anti-IGF2BP3 antibody compared to IgG in HCCL-M3 and Huh-7 cells. (h-i). qRT-PCR assay showing MCM10 mRNA stability in HCCL-M3 cells. (j) The effect of silencing HuR on MCM10 expression in HCCL-M3 and Huh-7 cells. (g-h). The expression of MCM10 protein under the presence of IGF2BP3 knockdown (g) or overexpression (h). All the data are presented as the mean ± standard deviation (n = 3). *P < 0.05, **P < 0.01, compared with the control group.
