Fig. 6

Downregulation of AC133552.2 markedly enhances osteosarcoma cell proliferation and migration while inhibiting apoptosis. (a,c) The expression of AC133552.2 was significantly reduced in the 143B and U2OS cell lines. (b,d,e,f) CCK-8 and EdU assays demonstrated that the knockdown of AC133552.2 markedly promoted the proliferation of 143B and U2OS cells. (g,h) Transwell assays indicated a significant increase in the migration capacity of 143B and U2OS cells following AC133552.2 knockdown. (i,j) Scratch assays further confirmed that the migration ability of 143B and U2OS cells was significantly enhanced after AC133552.2 knockdown. (k,l) Apoptosis assays revealed a substantial decrease in the apoptosis rate of 143B and U2OS cells following the knockdown of AC133552.2. The data were presented as the mean ± SD with n = 3. ***p < 0.001, **p < 0.01, *p < 0.05.