Fig. 4

Platelet endothelial cell adhesion molecule (CD31) protein expression in the fast-twitch tibialis anterior (TA) and slow-twitch soleus (Sol) muscles in the sedentary and trained groups of young (Y) and middle-aged (M) mice. The content of CD31 (n = 14–15–17–14) in the TA (A) and Sol muscles (n = 14–14–14–14) (B). The data are presented as the means + SDs. Each data point in the dot plot represents one individual mouse sample. Two-way ANOVA followed by Tukey’s post hoc test was used. ns, not statistically significant. The representative blots and corresponding Ponceau detection results are shown in Supplemental Figure S6.