Fig. 5
Pro-angiogenic genes expression in the fast-twitch extensor digitorum longus (EDL) muscle (normalized to a geometric mean of four reference genes) in the sedentary and trained groups of young (Y) and middle-aged (M) mice. The expression of vascular endothelial growth factor A (Vegfa) (n = 15–15–17–14) (A); FMS-like tyrosine kinase 1 (Flt1) (n = 15–15–16–14) (B); hypoxia-inducible factor 1, alpha subunit (Hif1a) (n = 14–15–17–14) (C); matrix metallopeptidase 14 (Mmp14) (n = 15–14–16–14) (D); nitric oxide synthase 1 (Nos1) (n = 15–15–17–14) (E); nitric oxide synthase 3 (Nos3) (n = 15–15–17–14) (F). The data are presented as the means + SDs. Each data point in the dot plot represents one individual mouse sample. Two-way ANOVA followed by Tukey’s post hoc test was used. Statistically significant changes (p < 0.05) are plotted on the graphs; ns, not statistically significant.
