Fig. 3
Growth of E. coli after transformation with various retroviral plasmids on LB/carbenicillin and LB agar plates. Heat-shock competent E. coli Stbl3 cells were transformed with either no plasmid DNA (mock) or with retroviral plasmids (pBabe Puro, pBMN-I-EGFP/Puro, pBMN-I-EGFP-2A-Puro, prBMN-I-EGFP-2A-Puro, pBMN-I-GFP and prBMN-I-EGFP) and streaked as shown in (a) onto (b) an LB agar plate containing 50 µg/mL carbenicillin or (c) an LB agar plate without antibiotics. Plates were incubated at 37 °C for 20 h, and images were captured. All plasmid transformations were performed in the same E. coli strain (Stbl3). Results are representative of three independent experiments.
