Fig. 5

Flow cytometric analyses for EGFP fluorescence in 38B9 cells infected with retroviral supernatants derived from pBMN-I-GFP and prBMN-I-EGFP. 38B9 cells were infected with retroviral supernatants from (a) mock-, (b) pBMN-I-GFP- or (c) prBMN-I-EGFP-transfected Platinum-E cells. EGFP fluorescence intensities, used to evaluate infection efficiency, were measured by flow cytometry on the day of infection, one day after infection, and every other day for 3 days. Data acquisition and gating strategy followed those described in Supplemental Fig. 11. EGFP fluorescence intensities of live, single cells are presented as overlay histograms, with relative cell numbers normalized and presented as %Max. The mean fluorescence intensities (MFIs) of the EGFP-positive populations are indicated next to the labels. The results are representative of two independent experiments, each performed using retroviral supernatant generated from independently isolated plasmids.