Fig. 6 | Scientific Reports

Fig. 6

From: Rhodiola crenulata induces apoptosis in bone metastatic breast cancer cells via activation of caspase-9 and downregulation of MtMP activity

Fig. 6

Apoptotic biomarkers experienced no significant changes when the healthy bone was treated with R. crenulata. RT-PCR experiments were performed with samples treated with R. crenulata for 24 h. Samples were treated with 800 ppm of R. crenulata. (A) Gene expression of bcl-2 is represented by 33-day hMSCs (bone) compared to the 3D culture of breast cancer cells (MM 231 and MCF-7). (B) Gene expression of p53 is represented by 33-day hMSCs (bone) compared to 3D BM culture of breast cancer cells (MM 231 and MCF-7). Significance (*) indicates the significance of relative expression between treated and non-treated cultures. *p < 0.05, **p < 0.01 ***p < 0.001. Samples were processed with GraphPad Prism software. (C) A schematic representation of the intrinsic apoptosis pathway being activated. Specifically, the role of caspase-9 in activating apoptosis.

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