Fig. 2
From: MB based RT-qPCR increase the clinical application of cfEBV DNA for NPC in non-endemic area of China
Comparing cfEBV DNA quantitation with nucleic acid extracted by MB and CP. (A-C) Nucleic acid extracted from the same plasma by CP and MB method, respectively (N = 60). The same amplification kit measured cfEBV DNA. Different cfEBV DNA loads were compared. Group 1: cfEBV DNA < 100 copies/ml (A); Group 2: 100 copies/mL ≤ cfEBV DNA< 400 copies/ml (B); Group 3: cfEBV DNA ≥ 400 copies/ml (C). (D) Digital PCR was used to replicate the outcomes of MB based RT-qPCR. Representative digital PCR amplification results of cfEBV DNA in samples (S1–S7) are displayed. (E) The relative significance of the two techniques was investigated (P < 0.0001, r = 0.9034). (F) Bland-Altman plots displayed the comparison between Sichuan Cancer Hospital and Sun Yat-Sen University Cancer Center. (G) The correlation analysis of two libraries.
