Fig. 1

Effect of astaxanthin on the oxidative stress of horse PBMCs. ROS accumulation in control cells (CTRL), oxidative stress-induced cells (OS) and oxidative stress-induced cells treated with astaxanthin at 10 µM (OS + ASTA10) or 20 µM (OS + ASTA20) was tested using the Muse Analyzer Flow Cytometry. (A) Flow cytometry histograms show the percentage of ROS-negative (blue, M1) and ROS-positive (red, M2) cells. (B) Bar graphs show the statistical analysis of all pairwise comparisons in ROS-negative and ROS-positive cells. Mean and standard deviation are given. Statistical significance is depicted as; *** p-value < 0.001; ns, not significant.