Fig. 3

Effect of astaxanthin on the gene expression of oxidative stress markers in horse PBMCs. (A–F) Analysis of the gene expression levels of selected oxidative stress markers (SOD1, NRF1, SOD2, CAT, NRF2, GPX) in control cells (CTRL), oxidative stress-induced cells (OS) and oxidative stress-induced cells treated with astaxanthin at 10 µM (OS + ASTA10) or 20 µM (OS + ASTA20) by qRT-PCR. Mean and standard deviation are given. Statistical significance is depicted as; * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001, **** p-value < 0.0001; ns, not significant.