Fig. 5 | Scientific Reports

Fig. 5

From: Multiparametric physicochemical analysis of a type 1 collagen 3D cell culture model using light and electron microscopy and mass spectrometry imaging

Fig. 5

SIMS images of sections prepared from the same HDF-seeded collagen scaffold after resin embedding. (a) Optical image of the face of the resin block (left), brightfield images of 250 nm thick sections on a silicon wafer (white arrows indicate ToF-SIMS sputter craters) (centre) and ToF-SIMS and NanoSIMS total ion images (right). (b) ToF-SIMS images of molecular fragments associated with the nuclear membrane, the nucleus, the plasma membrane and/or cytosol, the scaffold and/or interstitium and the resin. (c) NanoSIMS images of oxygen, cyanide and phosphorus ions in the same cell. All sections were prepared from the same sample for which optical microscopy images are shown in Fig. 3a.

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