Fig. 7 | Scientific Reports

Fig. 7

From: Multiparametric physicochemical analysis of a type 1 collagen 3D cell culture model using light and electron microscopy and mass spectrometry imaging

Fig. 7

Overview of sample preparation steps. Collagen gels seeded with HDFs were cast on gridded coverslips and stained for fluorescence microscopy. A silicone spacer ring was added before the sample was encapsulated under a second coverslip. For SIMS and TEM analysis additional contrast agents were added before resin embedding and sectioning.

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