Fig. 2
From: Plakoglobin does not participate in endothelial barrier stabilization mediated by cAMP
Effect of PG deficiency on central structural and signalling molecules, cAMP levels and small Rho GTPases activity. (a) Representative Western blot, N ≥ 5 and (b) PCR, N ≥ 3 analyses of junctional and junctional-related molecules revealing the protein and mRNA expression levels, respectively, in WT and PG-KO cells. α-tubulin was used as loading control for WB. Equal loading for the PCR analyses was validated by B2M expression. (c) Bar diagram depicting the relative protein expression of each analysed target of interest in PG-KO when compared to WT cells. (d) Bar diagram presenting the mRNA expression in PG-KO, relative to WT cells. (e) Western blot analysis illustrating the relative expression of signaling molecules such as Epac1, Rac1 and RhoA upon PG depletion with respective α-tubulin loading control; N ≥ 6 (f) Bar diagram representing the protein expression of the targets tested in (e), relative to WT, after densitometric measurements of the signal intensity were accomplished. (g) Bar graph illustrating the activity of Rac1 and RhoA GTPases measured by G-LISA; N ≥ 7 h) Levels of intracellular cAMP in untreated WT and PG-KO, detected by ELISA; N = 6. Data are presented as mean ± SEM; the following values were considered significant * p ≤ 0,05; ** p ≤ 0,01; **** p ≤ 0,0001.
