Fig. 6
From: Plakoglobin does not participate in endothelial barrier stabilization mediated by cAMP
Expression pattern of structural proteins and VE-cadherin-based complex composition in WT and PG-KO cells before and after cAMP elevation. (a) Representative Western blots in WT and PG-KO cells, showing the protein expression of VE-cadherin, β-catenin, ZO-1 and cortactin upon cAMP elevation. α-tubulin was used as a loading control; N ≥ 3. (b) Bar diagrams illustrating the densitometric analysis from (a) in WT and PG-KO cells, respectively. The bars reflect the protein abundance in F/R treated cells, when compared to cells exposed to Vehicle. (c) VE-cadherin pull-down in WT and PG-KO cells treated either with Vehicle or F/R;phosphorylation status of VASP confirmed effective treatment with F/R; Bands originated from the same membrane, but with different exposure time applied, were separated with a vertical black line. Original blots are presented in Figure S8 a, provided in the Supplementary info file; N = 6. Data are presented as mean ± SEM; unpaired t-test; * p ≤ 0,05; ** p ≤ 0,01; **** p ≤ 0,0001.
