Table 1 List of primary antibodies.
From: Plakoglobin does not participate in endothelial barrier stabilization mediated by cAMP
Antibody | Species | Cat Number | Dilution Rate/Purpose | Company |
|---|---|---|---|---|
VE-cadherin | Rabbit | ab33168 | 1:1000-WB* | Abcam |
VE-cadherin Tyr658 | Rabbit | 44-1144G | 1:1000- WB* 1:100-IF# | Thermo Fisher Scientific |
VE-PTP | Mouse | 610180 | 1:500- WB* | BD Transduction Laboratories |
VE-PTP | Mouse | NBP2-67087 | 1:50-IF# | Novus Biologica |
α-Tubulin | Mouse | ab7291 | 1:1000- WB* | Abcam |
ZO-1 | Rabbit | 617300 | 1:1000- WB* 1:100- IF# | Thermo Fisher Scientific |
β-catenin | Mouse | 61054 | 1:1000- WB* 1:100- IF# | BD Transduction Laboratories |
Plakoglobin | Mouse | 61005 | 1:1000- WB* 1:50- IF# | Progen |
PG Ser665 | Mouse | house made | Pure supernatant | |
vWF | Rabbit | A0082 | 1:1000- WB* 1:100- IF# | Dako |
PECAM-1 | Mouse | sc-37676 | 1:500- WB* 1:50- IF# | Santa Cruz |
Rac1 | Mouse | 610651 | 1:1000- WB* | BD Transduction Laboratories |
RhoA | Rabbit | 10749-1-AP | 1:1000- WB* | Proteintech |
Occludin | Rabbit | 40–4700 | 1:1000- WB* | Thermo Fisher Scientific |
Cortactin | Mouse | BDL-868102 or 05-180-AF647 | 1:1000- WB* 1:100-IF# | Biozol or Millipore |
Epac1 | Rabbit | ab109415 | 1:1000- WB* | Abcam |
VASP/p-Vasp 157 | Rabbit | 0012 − 02 | 1:1000- WB* | ImmunoGlobe |
Claudin-1 | Mouse | 37–4900 | 1:500 | Thermo Fisher Scientific |
