Fig. 2

S225 phosphorylation is independent of other phosphorylation events in LCSI. Huh7 cells were electroporated with the indicated mJFH-1 WT and mutant RNAs. (a) Lysates were analysed by western blotting for pS225, total NS5A and α-actin. (b) Quantification of pS225 (red) abundance from multiple western blots (n = 3). Values are mean ± SE. **** P > 0.0001.