Fig. 2

tet2^−/−;tet3^−/− retinae show alterations in cell type compositions during development. (A) Differentially abundant cell types at 36,48,72, 120hpf (p < 0.05, FDR < 0.1). (B) Immunofluorescent staining of PKCα⁺ BCs at 120hpf. Nuclei stained with DAPI (white). PKCα (magenta). Scale bar = 50 µm. (C) Quantification of proportion of PKCα⁺ BCs relative to all DAPI⁺ cells across the retina (p = 1.59 × 10^–2). (D) Quantification of proportion of PKCɑ⁺ BCs relative to DAPI⁺ cells localized to the inner nuclear layer (p = 1.59 × 10^–2). (E) Proportion of nuclei localized to the inner nuclear layer in sibCTL and tet2^−/−;tet3^−/− retinae (p = 3.43 × 10^–1). (F) Proportion of nuclei localized to the ganglion cell layer in sibCTL and tet2^−/−;tet3^−/− retinae (p = 3.43 × 10^–1). (G) Proportion of nuclei localized to the outer nuclear layer in sibCTL and tet2^−/−;tet3^−/− retinae (p = 4.13 × 10^–1). For D-H, sibCTL N = 4; tet2^−/−;tet3^−/− N = 5.