Fig. 4
From: tet2 and tet3 regulate cell fate specification and differentiation events during retinal development
BC formation is disrupted in tet2−/−;tet3−/− retinae (A–C) UMAP projections of pooled 120hpf sibCTL and tet2−/−;tet3−/− BCs indicating (A) BC type: BC-ON, BC-OFF or BC_early; (B) BC subpopulation and (C) imbalance score. (D) BC subpopulation distributions in sibCTL and tet2−/−;tet3−/− retinae at 120hpf. Cluster 13, which shows a disproportionately high composition of tet2−/−;tet3−/− cells, is annotated. (E) Differential abundance analysis on 120hpf BC subpopulations. (F) UMAP projection of 120hpf BCs colored by subpopulations that show disproportionately more tet2−/−;tet3−/− cells (gold), sibCTL cells (purple), or no difference (gray). (G) Median AUCell scores for neuronal GO Terms across 120hpf BC subpopulations. GO Terms assayed include cell projection (GO:0042995) synapse (GO:0045202) transmembrane transporter (GO:0015318) calcium ion binding (GO:005509) and gated channel activity (GO:0022836). Statistics comparing AUCell scores across 120hpf BC clusters are available in Table S8. NS = not significant. (H) UMAP projection of 120hpf BCs colored by AUCell scoring for the synapse GO term (GO:0045202).
