Fig. 1 | Scientific Reports

Fig. 1

From: Identification of mechanistic CKD biomarkers in a rat SNx kidney fibrosis model by transcriptomics and proteomics detectable in biofluids

Fig. 1

Characterisation of rat SNx study samples. (a) The surgical procedure in rats termed sub-total nephrectomy (SNx) where 5/6 of the functioning kidneys are removed and post-surgery the animals develop failing kidney function from day 50 onwards, depicted on a timeline with monitoring of the failing kidneys using a combination of serum creatinine and proteinuria measurements. (b) Study 1 kidney sections from either sham (n = 3) or SNx rats (n = 3), stained with Picrosirius Red (PSR) and the PSR-stained kidneys were scanned, the percentage of PSR area plotted alongside the other functional kidney readouts, Proteinuria, Serum creatinine and Protein creatinine ratio. Scale bar: 200 µm. (c) Study 2 kidney sections from 1/4 sham rats or 2/6 SNx rats, stained with PSR and PSR-stained kidneys from all rats were scanned, percentage of PSR area plotted alongside the other functional kidney readouts, Proteinuria, Serum creatinine and Protein creatinine ratio. Scale bar: 4 mm or 500 µm, as indicated.

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