Fig. 1 | Scientific Reports

Fig. 1

From: Developmental differentiation of mouse inner ear neuron subpopulations resolved with a peripherin-promoter reporter within the Grm8 locus

Fig. 1

Prph promoter (Prphp) driven - mCherry transgene cassette design and creation of mouse lines. (A) The mCherry reporter is flanked by an upstream P1 Prphp element and a downstream P2 Prphp repressor element29. The construct included the CBA (Chicken β-actin) promoter, KOZAK sequences, the human Diphtheria Toxin Receptor (hDTR) coding sequence element, expression regulatory domains and regulatory sequences including: CBA exon 1, 2, CBA intron, KOZAK sequences, IRES (internal ribosome entry site), WHP (Woodchuck Hepatitis Virus), WPRE (Posttranscriptional Regulatory Element), BGHpA (bovine growth hormone poly A). (B) PCR genotyping of the transgene as shown in (A) exhibited mCherry expression in the dorsal root ganglion (shown for Line 43 in C).

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