Fig. 3
Prphp-mCherry transgene integration inside Grm8 on chromosome 6. (A) Schematic of the integration of the Prphp-mCherry transgene within the Grm8 gene. The mutant Grm8 disrupted by Prphp-mCherry shows 2½ copies of the transgenic construct integrated 4 kb from the N-terminus coding region of Grm8. The black arrows indicate the direction of the reading frame, Grm8 is transcribed right to left, while the transgene is transcribed left to right. Within the Grm8 gene, 200 kb were deleted by the transgene integration, resulting in the deletion of exon 1 (yellow). This disruption extended into exon 2. Downstream exon 5 was also deleted (orange). Duplications of exons 2, 3 and 4 (pink) are noted. This schematic is modelled on the Grm8 201 isoform; given the Prphp-mCherry transgene integration in the Grm8 N-terminus region, alternative splicing of Grm8 isoforms, which are clustered in the C-terminus, have no bearing on the mutated protein structure. (B) The transgene is concatenated and includes two full copies. The contig size is 1,830,475 bp, derived from Oxford Nanopore Technologies sequencing.
