Fig. 4

AlphaFold2 modelling of mutated mGluR8 associated with Prph_p-mCherry transgene integration. AlphaFold 2.0 - based structural modelling of the effects of the exon deletions and duplications in Grm8, produced by the Prph_p-mCherry transgene integration in mouse Line 43. The dimerized mutant (A) mGluR8 protein shows pronounced structural differences at the glutamate binding site (Venus fly trap) domain. Pink highlighting delineates amino acid affected by duplicated exons; blue represents unchanged amino acid domains. The difference in steric effects has caused the disulphide bridge (9 cysteines domain) to be further apart than in the native mGluR8. The 7 transmembrane domain regions are equivalent for the native and mutated protein isoforms. The native (B) Grm8 gene shows the N-terminal region sites lost by integration of the Prph_p-mCherry transgene (deleted exons; yellow and orange). Visualised with Pymol (Ver 2.5.0). Videos of the mutant Grm8 dimer and native dimer are provided as Supplementary Video 1.