Fig. 7
Development of SGN neurites in the basal cochlea of the Prphp-mCherry mouse. (A–D) Peripherin (white) and mCherry (red) immunofluorescent staining in postnatal day 1, 7, 14 and 30 cochleae intact samples. These samples are oriented to focus on the hook region of the spiral ganglion. SGN cell bodies reside in Rosenthal’s canal (RC), their fibers project to the two hair cell populations via the osseous spiral lamina (OSL). Fibers travelling below the hook region are projecting from the vestibular ganglion to the posterior RC. (E, E′, E″) Illustrate the combination of radial fiber co-labeling (IHC target) and the outer spiral fibre co-labeling (OHC target). PEGASOS / Lightsheet / Imaris processing with non-specific immunolabeling manually reduced in the background of these images.
