Fig. 1
From: Development of a novel technology for long-term culture and live imaging of excised human tissue
Comparison of changes over time in skin tissue with each culture method. (a) Fluorescent immunostaining images of skin tissue after 0, 5, and 11 d of culture. Day 0 represents the state immediately after extraction and before culturing, serving as the control. The traditional method (upper row) and microneedle method (lower row) are displayed. In skin tissue cultured using traditional methods, the epidermis and dermis become exfoliated. The white asterisk on Day 11 indicates the region where the epidermis and dermis of the traditionally cultured skin tissue have peeled away. Each white bar indicates a scale of 100 μm. The image on the right provides a magnified view of the white frame in the Day 5 images. The nuclei of epidermal cells in traditionally cultured skin tissue appear smaller and nearly perfectly circular. Blue: nucleus; green: tropoelastin; red: COL VII. Each white bar indicates a scale of 50 μm. (b) TUNEL staining of skin tissue after 0, 5, and 11 d of culture. Day 0 represents the state immediately after extraction and before culturing, serving as the control. The traditional method (upper row) and microneedle method (lower row) are displayed. In skin tissue cultured using traditional methods, the epidermis and dermis become exfoliated. Blue: nucleus; red: TUNEL. Each white bar indicates a scale of 50 μm. (c) Concentrations of LDH and inflammatory cytokines in the culture medium supernatant following skin tissue culture. Gray line: traditional method; black line: microneedle culture. Bars represent S.D. (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001.
