Fig. 3
From: Development of a novel technology for long-term culture and live imaging of excised human tissue
Velocity analysis of the retrograde flow of epidermal cells at the epidermis–dermis junction. (a) Overall image of the epidermis–dermis boundary at 0, 1, 2, 3, and 4 h (Video 2). Blue indicates the nucleus, green shows the autofluorescence of elastin fibers (high intensity) and collagen fibers (low intensity), and red represents the cell membrane. A white bar indicates a scale of 5 μm. (b) A kymograph, created by isolating the white-framed area in the overall image, converts the cell membrane (red) to grayscale and arranges it chronologically. The retrograde flow velocity is calculated based on the angle of the diagonal line.
