Table 1 Primer sequences, amplicon sizes, and PCR conditions of the Escherichia coli virulence genes.

From: Public health threat of antimicrobial resistance and virulence genes in Escherichia coli from human-chicken transmission in Egypt

Reference

Target gene

Primer sequence (5ʹ–3ʹ)

Amplicon size (bp)

PCR conditions

35

Iss

F:

ATGTTATTTTCTGCCGCTCTG

R:

CTATTGTGAGCAATATACCC

266

initial denaturation of 5 min at 94 ◦C followed by 35 cycles, denaturation at 94 ◦C for 30 s, annealing at 54 ◦C for 30 s, extension at 72 ◦C for 30 s, and final extension at 72 ◦C for 10 min.

48

papC

F:

GACGGCTGTACTGCAGGGTGTGGCG

R:

ATATCCTTTCTGCAGGGATGCAATA

350

initial denaturation of 5 min at 94 ◦C followed by 30 cycles, denaturation at 94 ◦C for 40 s, annealing at 65 ◦C for 40 s, extension at 72 ◦C for 1 min, and final extension at 72 ◦C for 10 min

49

vgrG1

F: GTATCTTCCAGAATGAGGAC

R:

CATGTTCATCACAGAAGATT

831

initial denaturation of 5 min at 94 ◦C followed by 25 cycles, denaturation at 94 ◦C for 30 s, annealing at 54 ◦C for 30 s, extension at 72 ◦C for 1 min, and final extension at 72 ◦C for 10 min

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