Fig. 6

Whole blood spiked with fluorescent beads side vs main outlets. a) µbeads spiked at a 5000 beads/mL. left, SSC vs FITC scatter plot showing no FITC + beads. Right, scatter plot of the retentate tube showing the presence of FITC + events indicating the green fluorescent beads. b) µbeads spiked at < 100 beads/mL, analysis done using fluorescent microscopy images (488 nm laser excitation) of a 200 µL drop of each tube obtained after filtration in the chip. Left, filtrate drop, absence of any fluorescent bead. Right, retentate drop, large bright beads among the filtered cells, scale bar: 200 µm c) recovery rate of beads from whole blood at 5000 bead/mL and at 100 beads/mL portraying 100% recovery rate.