Fig. 7
From: Isolation of adipose stromal cells from blood using a two-step microfluidic platform ASCfinder
Filtration experiments of ASC – doped blood a) Schematic depicting the work-flow of the filtration and analysis experiments of fluorescent ASC from whole blood samples. b) ASC filtration from whole blood samples. Left, SSC vs FITC density plots of the filtrate tube (side-outlet), depicting absence of any GFP + event in the preselected gate, indicating absence of fluorescent ASC. Right, SSC vs FITC density plots of the retentate tube, with the GFP + fluorescent ASCs highlighted in the gate. c) RBC depletion and ASC recovery rates as a function of inlet flow rate. ASC recovery is not impacted by the flow rate in the HDF chip with > 96% ASC recovered. Retentate purity is impacted on the other hand, with a decline in RBC depletion rate reaching 95% at 13 µL/min.
