Fig. 3
Treatment with doxorubizen induces cell apoptosis The cells were stained with Annexin V and 7AAD, followed by Muse® Cell Analyzer analysis. A. The histograms represent the mean number of apoptotic cells (annexin V-positive cells) ± SEM from at least 3 experiments following 48 h of treatment with doxorubizen or dox (50 nM each) compared to the untreated cells; *p < 0.05. SEM: standard error of the mean. UT: untreated. B. Representative apoptosis analysis of BV-173 cells exposed to indicated treatments. The lower left quadrant represents the percentage of viable cells (negative for Annexin and 7AAD), the upper left quadrant represents necrotic cells (7AAD positive, annexin negative), the lower right quadrant represents cells in early apoptosis (annexin positive, 7AAD negative), and the upper right quadrant represents cells that are in late apoptosis (annexin and 7AAD positive). Similar gating strategy and analysis was utilized for the additional cell lines.
