Table 2 Oligonucleotide primers for allele-specific PCR and RT-PCR.
Target | Forward primer (5’-3’) | Reverse primer (5’-3’) | Amplicon size |
|---|---|---|---|
Endogenous NTN1 (exon 1) | TGGATGTCGTTGTTCTGTTCC | CTCGAACTTCTTGCCGAGGG | 727 nt |
NTN1-T2A-eGFP | TGAGCGCAATGTCACTCCTT | GCTGAACTTGTGGCCGTTTA | 533 nt |
eGFP | AAGAACGGCATCAAGGTGAA | CTCAGGTAGTGGTTGTCGG | 134 nt |
GAPDH | GGTGAAAGTCGGAG | GACACCCATCACAAACA | 397 nt |
