Fig. 2
CVB1-VLPNo−Tween80 and CVB1-VLPTween80 characterization, stability and binding capacity to EGCG. SDS-PAGE and Western Blotting of purified (A) CVB1-VLPNo−Tween80 and (B) CVB1-VLPTween80. The left panel in A and B shows the stainfree total protein staining of the purified CVB1-VLPNo−Tween80 and CVB1-VLPTween80. In the right panels of A and B VP1 and VP3 capsid proteins were detected by Western blot stained with a polyclonal anti-CVB1-6 rabbit antibody. VLP capsid proteins are indicated with arrows. B) Transmission electron micrographs of the negatively-stained purified (C) VLPNo−Tween80 and (D) CVB1-VLPTween80 with 25 000x magnification. Dynamic Light Scattering (DLS) analysis of (E) CVB1-VLPNo−Tween80 and (F) CVB1-VLPTween80 stored at room temperature. DLS analysis of (G) CVB1-VLPNo−Tween80 and (H) CVB1-VLPTween80 incubated with EGCG at room temperature.
