Fig. 5
From: A feasibility of computational drug screening for Fuchs endothelial corneal dystrophy
Morphological evaluation of drug candidate effects on TGF-β2–induced cell death in FECD model cells. Phase-contrast microscopy analysis of iFECD cells following drug treatment. Cells were pretreated with LDN193189 (1 µM), cercosporin (100 nM), or menadione (1 µM) for 24 h, followed by co-treatment with TGF-β2 (10 ng/mL) for 24 h. TGF-β2 treatment alone induced significant cell death, as evidenced by increased numbers of floating cells. Cotreatment with either LDN193189 or cercosporin markedly reduced TGF-β2-induced cell death, while menadione exacerbated cell death and induced widespread cell detachment, indicating cytotoxicity. Note that while LDN193189 and menadione were tested at 1 µM, cercosporin showed cytotoxicity at this concentration; therefore, a lower concentration of 100 nM was used based on preliminary studies demonstrating higher efficacy with reduced toxicity at this dose. Representative images from three independent experiments are shown. Scale bar: 100 μm.
