Fig. 1

DNA quantification method. (a) Schematic representation of the DNA quantification method for estimation of NA losses at the binding and elution steps in a sample preparation protocol. This quantification method was used to estimate % DNA bound and % DNA eluted for the different binding and elution factors tested during SHIFT-SP protocol development. (b) Standard curves used in the DNA quantification method. Two types of standard curves are used in this study. The curve generated with standards made in diluted LBB is used to estimate % DNA bound whereas the curve generated using standards made in 1X TE is used to estimate % DNA eluted. The standard curve “STD in 1:500 LBB” is similar to the standard curve “STD in 1X TE”, showing that dilution reduces the background effect of guanidine and triton in LBB.