Fig. 2

Binding condition determination. (a) Effect of binding time and pH on DNA binding efficiency. The figure shows a comparison of percent DNA bound to the beads when incubated with low and high pH LBB with increasing duration of binding, demonstrated for 100 ng. The percent DNA bound to beads is higher at low pH at all the time points tested. Experiments were performed using “orbital shaking” of tubes with DNA/bead/LBB mix. (b) Effect of binding method on DNA binding efficiency. The graph indicates the percent DNA bound by “orbital shaking” method and non-orbital “tip-based” method at 1 min incubation time, for a 100 ng and 1000 ng input DNA. For both quantities of input DNA, the amount of DNA bound by “tip-based” method is higher compared to “orbital shaking” method. The error bar in the graph depicts the SD. n = 6 for each condition in a and b.