Fig. 6
From: A rapid and high-yield method for nucleic acid extraction
SHIFT-SP used to extract DNA from enriched whole blood samples. (a) Schematic representation of the workflow details. Enriched whole blood is used as an input to SHIFT-SP protocol. DNA extracted from SHIFT-SP is amplified and sequenced by whole genome sequencing and targeted metagenomic sequencing workflows (b) Whole genome sequencing results correlated to E. coli and C. albicans present at 5 CFU/mL (c) Targeted genomic sequencing results shown for both 16s rDNA and ITS regions correlating with E. coli and C. albicans respectively. SHIFT-SP method enabled the extraction and detection of very low microbial DNA quantities from enriched blood samples.
