Fig. 1 | Scientific Reports

Fig. 1

From: HIF-1 regulates mitochondrial function in bone marrow-derived macrophages but not in tissue-resident alveolar macrophages

Fig. 1

HIF-1α deletion alters transcriptome both at baseline and following treatment with a HIF-1α stabilizer in BMDMs but only after HIF-1α stabilizer in TR-AMs. TR-AMs (A–C) and BMDMs (D–F) were cultured overnight (16 h) in the presence or absence of FG-4592 (25µM). (A, D) Western blot analysis of nuclear extracts to verify successful HIF-1α knockout in our transgenic system. (B, E) Clustering of samples based on RNA-seq gene expression profiles using t-SNE projection (C, F) Heatmap analysis of CHEA (ChIP-X Enrichment Analysis) HIF-1α transcription factor targets.

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