Fig. 3
HIF-1α deletion boosts mitochondrial function in BMDMs but has limited impact on TR-AM mitochondrial function. TR-AMs (A, B) and BMDMs (C-E) were cultured overnight (16 h) in the presence or absence of FG-4592 (25µM). (A, C) Mitochondrial stress test to measure oxygen consumption rate (OCR) using Seahorse XF24 in TR-AMs and BMDMs. Macrophages were treated sequentially with oligomycin (ATP synthase inhibitor), FCCP (uncoupler) and Rotenone/Antimycin A (complex I and III inhibitor, respectively). (B, D) Interleaved scatter plots quantifying mitochondrial respiration parameters. Data represents at least 3 experiments (n = 4 separate wells per group). Mitochondrial parameters were compared against Hif1a+/+ no treatment group. Significance was determined by two-way ANOVA with Bonferroni’s post test. (E) GC-MS metabolite analysis of BMDMs. Data represent at least 3 independent experiments (n = 3 replicates per experimental condition). All groups compared against Hif1a+/+ no treatment group with significance determined by two-way ANOVA with Bonferroni’s post test. All error bars denote mean ± SD. *, p < 0.05.
