Fig. 3
From: Oxygen level alters energy metabolism in bovine preimplantation embryos
Visual demonstration of the embryonic genome activation (EGA) in bovine embryos. (A) The track shows the transcriptional changes of embryos grown under hypoxia. The top left graph shows the differences between 8-cell stage embryos and zygotes demonstrating the first downregulation of the maternal genes (brown). On the right, we see the comparison of 16-cell embryos to zygotes, and we can see the major EGA wave in bovine, where the majority of genes is downregulated (brown) and first upregulation of the newly formed embryo transcripts appears (green). Nonsignificant changes are shown in grey. (B) The track shows the transcriptional changes of embryos grown under normoxia. Bottom left graph presents comparison of 8-cell stage embryos against zygotes, with first downregulation of genes (brown), while the right graph demonstrates the transcriptomic changes in 16-cell stage embryos against zygotes. Downregulated genes are in brown, nonsignificant in grey, and upregulated genes in green. For the genes to be significantly downregulated or upregulated, the FDR of 5% was used.
