Table 2 Stability ranking of candidate genes assessed using GeNorm, NormFinder, bestkeeper, and reffinder in mixed tissue samples.
From: Screening and validation of optimal real-time PCR reference genes for Abelmoschus Manihot
Rank | geNorm | NormFinder | BestKeeper | RefFinder | |||||
|---|---|---|---|---|---|---|---|---|---|
Gene | Stability | Gene | Stability | Gene | SD | CV | Gene | Stability | |
1 | PP2A1 | 0.58 | eIF | 0.15 | PP2A1 | 0.42 | 1.65 | eIF | 1.86 |
2 | PP2A2 | 0.58 | GTP | 0.44 | PP2A2 | 0.44 | 1.81 | PP2A1 | 1.86 |
3 | EIF4α | 0.67 | PP2A1 | 0.49 | eIF | 0.63 | 2.75 | PP2A2 | 2.11 |
4 | eIF | 0.69 | EIF4α | 0.49 | EIF4α | 0.68 | 3.01 | EIF4α | 3.46 |
5 | ACT2 | 0.75 | PP2A2 | 0.51 | GTP | 0.71 | 2.85 | GTP | 4.16 |
6 | GTP | 0.78 | ACT2 | 0.62 | ACT2 | 0.72 | 3.48 | ACT2 | 5.73 |
7 | PHD | 0.92 | PP2A3 | 1.11 | PP2A3 | 0.85 | 3.22 | PP2A3 | 7.45 |
8 | vps | 1.03 | vps | 1.25 | PHD | 0.88 | 3.51 | PHD | 8.21 |
9 | PP2A3 | 1.11 | PHD | 1.32 | vps | 0.99 | 3.99 | vps | 8.24 |
10 | YLS | 1.23 | YLS | 1.54 | YLS | 1.15 | 4.86 | YLS | 10.00 |
11 | TUA | 1.56 | TUA | 2.97 | TUA | 2.49 | 10.59 | TUA | 11.00 |
