Fig. 4
From: Differential biomarker expression of blood and lymphatic vasculature in multi-organ-chips
Distinct biomarker profiles of BECs and LECs remain unchanged with lymphatic and blood flow. (A) Principal component analysis of 16 biomarkers detected in supernatants of BECs (red) and LECs (blue) cultured under blood (B) or lymphatic (L) flow for 7 days (outlined) or 14 days. (B) Quantification of pro-inflammatory markers IL-6, sICAM-1 and LIGHT, (C) angiogenic markers TIE2 and PlGF and (D) anti-angiogenic markers/homeostatic markers sST2, sFLT1 in BECs or LECs cultured for 7 or 14 days under blood (B) or lymphatic (L) flow. PC: principal component. The data represent mean ± SEM; n = 3 with 2 or more intra-experimental replicates (except repeat 1 BEC, blood flow, day 14); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; 2-way ANOVA. Symbols in bar graphs represent different experimental repeats with different donors (● repeat 1, ■ repeat 2, ▲ repeat 3).
